Fl83b cells
WebOriginal magnification: 100×. (C) FL83B cells were transfected with HA-Daxx and then treated with or without TGF-β for 24 h. Western blotting analysis of Daxx, E-cadherin and Vimentin expression. Relative expression was normalized to β-actin expression as a reference. The graph shows quantitative densitometry from Western blotting. WebMay 21, 2024 · Abstract. FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its …
Fl83b cells
Did you know?
WebApr 1, 1973 · FL83B is a normal cell line of hepatocytes from a C57BL/6J mouse of 15–17 days of gestation (Breslow et al., 1973). FL83B required F-12 K medium with 10% FBS. … Web(F) Lipid content analysis of FL83B cells treated with 400 mM OA and different CLH concentrations by using Nile red stain. The data are given as mean ± SEM (n ¼ 6).
WebApr 1, 1973 · The FL83B cells were not disrupted to determine whether VLDL was present intracellularly. In summary, our data show that the FL83B mouse liver cell line grows in a … WebJan 15, 2024 · FL83B cells were induced as fatty liver cell model by 0.5 mM oleic acid for 48 h, and treated with various concentration of fucoxanthin for 24 h. The results demonstrated that fucoxanthin significantly suppressed lipid accumulation and decreased lipid peroxidation in hepatocytes.
WebFL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal … WebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot …
WebDec 29, 2015 · FL83B cell line represent normal hepatocytes whereas hHSC cell line has the angiotensin 1 receptors which is the main target for losartan receptors. Confocal …
WebThe short-and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture system were determined (Fig. 1A), which could monitor cellular morphology ... early orthodontic treatment minnetonkaWebFL83B cells were obtained from the Taiwan Bioresource Collection and Research Center (BCRC number: 60325) and cultured in F12K medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified atmosphere with 5% CO 2. To make the stock medium containing 4 mM OLA, sodium oleate was conjugated … early oscar winner jannings crossword clueWebDec 1, 2024 · Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF- α -treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p -coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was p -coumaric … early oscar predictions 2023WebFeb 6, 2013 · FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent d-glucose derivative, was performed, … early or late springWeb2.3. FL83B cell culture First, the culture method of FL83B cells was slightly modified from the American Type Culture Collection (ATCC) [18]. The short- and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture sys-tem were determined (Fig. 1A), which could monitor cst time winterWebAug 4, 2024 · Cytotoxicity of PG/Dox synergism against normal cells. To access putative toxicity from PG/Dox synergism in liver, kidney, and heart, FL83B (murine normal hepatocyte), HK-2 (human kidney proximal tubule epithelial cell), and h9c2 (murine normal cardio-myoblast) were sequentially treated with PG and Dox for 12 h, respectively. early orthodontic treatment lutherville mdWebJul 13, 2024 · FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non-targeting agRNA (control) and Tcf4 or Mafk sgRNA. early origins of christianity