WebOrganelle Reagent III contains a mixture of GA-selective, ER-selective and nucleus-selective dyes suitable for live cell staining. Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the Reagent III is more … WebApr 1, 2001 · The nuclear localization signal (NLS) from SV40 attached to GFP allowed production of transgenic plants with green fluorescent nuclei (Fig. 1B) but a detectable amount of this smaller GFP fusion protein remains in the cytoplasm (RH Köhler and MR Hanson, unpublished results).
Probes for Fluorescent Visualization of Specific Cellular Organelles
WebGolgi-Cox staining is a widely used method for characterizing axon and dendritic morphology and several attempts to combine this technique with immunofluorescence and transmission electron microscopy have been proposed. With few exceptions, most of the protocols were characterized by a high degree of complexity and low reproducibility. WebUnique green fluorescent dye. CF® Dye Spectrum: CF®350 Dye. CF®350 is a blue fluorescent dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF350. Technical Summary. Abs/Em Maxima: 347/448 nm Extinction coefficient: 18,000 Molecular weight: ~ 496 Excitation source: UV Replaces: Alexa Fluor® 350, AMCA, graphics tablet perspective
Fluorescent Dyes Science Lab Leica Microsystems
WebAug 1, 2012 · The fluorescent probes used to tag the Golgi make use of this function for labeling by using a fluorescent ceramide. In this protocol, two probes for labeling the Golgi are presented: a classic NBD-ceramide and a BODIPY-ceramide probe that is more resistant to photobleaching. MeSH terms 4-Chloro-7-nitrobenzofurazan / analogs & … WebWheat germ agglutinin (WGA) conjugates are commonly used as markers of the trans-Golgi. Fluorescent conjugates of concanavalin A (Con A) also stain the Golgi but with … WebBODIPY-BEA localized predominantly in the endoplasmic reticulum (ER) and Golgi complex of viable cells and was extracted by detergent treatment, suggesting it interacts … chiropractors blend muscle ezze pm